data partial cytochrome b sequences Search Results


nadph dependent cytochrome p450 reductase cpr  (Thermo Fisher)
98
Thermo Fisher nadph dependent cytochrome p450 reductase cpr
Nadph Dependent Cytochrome P450 Reductase Cpr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 1 article reviews
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gene exp cyp7a1 mm00484150 m1  (Thermo Fisher)
94
Thermo Fisher gene exp cyp7a1 mm00484150 m1
Expression of Zone 1 and Zone 3 hepatocyte markers and function in D‐Hep cells. (a) FHS‐DFAT cells were cultured in hepatic medium for 14 days. The temporal expression of Zone 1 hepatocyte marker genes (Oct and Pck1) was examined by real‐time PCR analysis in D‐Hep cells during hepatic differentiation. (b) The temporal expression of Zone 3 hepatocyte marker genes (Cyp2a5, Cyp1a2, Cyp2e1, <t>Cyp7a1</t> and Lgr5) was examined using real‐time PCR analysis in D‐Hep cells during hepatic differentiation. Mean values ± SEM . were normalized to Gapdh and expressed relative to normal hepatocytes from adult mice. Superscript letters indicate significant ( p < .01) differences. p ‐Value was calculated using Tukey's honestly significant difference test. (c) Schematic diagram of the experimental procedure. (d) The cell viabilities of DFAT cells (blue), D‐Hep cells (red) and primary hepatocytes (black) were assessed by ATP assay after a 48‐hr exposure to the three compounds (acetaminophen, tamoxifen and troglitazone). p ‐Value was calculated using Student's t test (** p < .01). NS, not significant. All scale bars represent 50 μm. All quantitative data are means ± SD . ( n = 3 experiments)
Gene Exp Cyp7a1 Mm00484150 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp cyp2a4 mm00487248 g1  (Thermo Fisher)
91
Thermo Fisher gene exp cyp2a4 mm00487248 g1
Expression of Zone 1 and Zone 3 hepatocyte markers and function in D‐Hep cells. (a) FHS‐DFAT cells were cultured in hepatic medium for 14 days. The temporal expression of Zone 1 hepatocyte marker genes (Oct and Pck1) was examined by real‐time PCR analysis in D‐Hep cells during hepatic differentiation. (b) The temporal expression of Zone 3 hepatocyte marker genes (Cyp2a5, Cyp1a2, Cyp2e1, <t>Cyp7a1</t> and Lgr5) was examined using real‐time PCR analysis in D‐Hep cells during hepatic differentiation. Mean values ± SEM . were normalized to Gapdh and expressed relative to normal hepatocytes from adult mice. Superscript letters indicate significant ( p < .01) differences. p ‐Value was calculated using Tukey's honestly significant difference test. (c) Schematic diagram of the experimental procedure. (d) The cell viabilities of DFAT cells (blue), D‐Hep cells (red) and primary hepatocytes (black) were assessed by ATP assay after a 48‐hr exposure to the three compounds (acetaminophen, tamoxifen and troglitazone). p ‐Value was calculated using Student's t test (** p < .01). NS, not significant. All scale bars represent 50 μm. All quantitative data are means ± SD . ( n = 3 experiments)
Gene Exp Cyp2a4 Mm00487248 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp cyp19a1 mm00484049 m1  (Thermo Fisher)
99
Thermo Fisher gene exp cyp19a1 mm00484049 m1
Expression of Zone 1 and Zone 3 hepatocyte markers and function in D‐Hep cells. (a) FHS‐DFAT cells were cultured in hepatic medium for 14 days. The temporal expression of Zone 1 hepatocyte marker genes (Oct and Pck1) was examined by real‐time PCR analysis in D‐Hep cells during hepatic differentiation. (b) The temporal expression of Zone 3 hepatocyte marker genes (Cyp2a5, Cyp1a2, Cyp2e1, <t>Cyp7a1</t> and Lgr5) was examined using real‐time PCR analysis in D‐Hep cells during hepatic differentiation. Mean values ± SEM . were normalized to Gapdh and expressed relative to normal hepatocytes from adult mice. Superscript letters indicate significant ( p < .01) differences. p ‐Value was calculated using Tukey's honestly significant difference test. (c) Schematic diagram of the experimental procedure. (d) The cell viabilities of DFAT cells (blue), D‐Hep cells (red) and primary hepatocytes (black) were assessed by ATP assay after a 48‐hr exposure to the three compounds (acetaminophen, tamoxifen and troglitazone). p ‐Value was calculated using Student's t test (** p < .01). NS, not significant. All scale bars represent 50 μm. All quantitative data are means ± SD . ( n = 3 experiments)
Gene Exp Cyp19a1 Mm00484049 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mannitol dehydrogenase rsmdh  (GenScript corporation)
90
GenScript corporation mannitol dehydrogenase rsmdh
Expression of Zone 1 and Zone 3 hepatocyte markers and function in D‐Hep cells. (a) FHS‐DFAT cells were cultured in hepatic medium for 14 days. The temporal expression of Zone 1 hepatocyte marker genes (Oct and Pck1) was examined by real‐time PCR analysis in D‐Hep cells during hepatic differentiation. (b) The temporal expression of Zone 3 hepatocyte marker genes (Cyp2a5, Cyp1a2, Cyp2e1, <t>Cyp7a1</t> and Lgr5) was examined using real‐time PCR analysis in D‐Hep cells during hepatic differentiation. Mean values ± SEM . were normalized to Gapdh and expressed relative to normal hepatocytes from adult mice. Superscript letters indicate significant ( p < .01) differences. p ‐Value was calculated using Tukey's honestly significant difference test. (c) Schematic diagram of the experimental procedure. (d) The cell viabilities of DFAT cells (blue), D‐Hep cells (red) and primary hepatocytes (black) were assessed by ATP assay after a 48‐hr exposure to the three compounds (acetaminophen, tamoxifen and troglitazone). p ‐Value was calculated using Student's t test (** p < .01). NS, not significant. All scale bars represent 50 μm. All quantitative data are means ± SD . ( n = 3 experiments)
Mannitol Dehydrogenase Rsmdh, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mannitol dehydrogenase pfmdh  (GenScript corporation)
90
GenScript corporation mannitol dehydrogenase pfmdh
Expression of Zone 1 and Zone 3 hepatocyte markers and function in D‐Hep cells. (a) FHS‐DFAT cells were cultured in hepatic medium for 14 days. The temporal expression of Zone 1 hepatocyte marker genes (Oct and Pck1) was examined by real‐time PCR analysis in D‐Hep cells during hepatic differentiation. (b) The temporal expression of Zone 3 hepatocyte marker genes (Cyp2a5, Cyp1a2, Cyp2e1, <t>Cyp7a1</t> and Lgr5) was examined using real‐time PCR analysis in D‐Hep cells during hepatic differentiation. Mean values ± SEM . were normalized to Gapdh and expressed relative to normal hepatocytes from adult mice. Superscript letters indicate significant ( p < .01) differences. p ‐Value was calculated using Tukey's honestly significant difference test. (c) Schematic diagram of the experimental procedure. (d) The cell viabilities of DFAT cells (blue), D‐Hep cells (red) and primary hepatocytes (black) were assessed by ATP assay after a 48‐hr exposure to the three compounds (acetaminophen, tamoxifen and troglitazone). p ‐Value was calculated using Student's t test (** p < .01). NS, not significant. All scale bars represent 50 μm. All quantitative data are means ± SD . ( n = 3 experiments)
Mannitol Dehydrogenase Pfmdh, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cyp2c19 gene sequence data  (Sequenom)
86
Sequenom cyp2c19 gene sequence data
Expression of Zone 1 and Zone 3 hepatocyte markers and function in D‐Hep cells. (a) FHS‐DFAT cells were cultured in hepatic medium for 14 days. The temporal expression of Zone 1 hepatocyte marker genes (Oct and Pck1) was examined by real‐time PCR analysis in D‐Hep cells during hepatic differentiation. (b) The temporal expression of Zone 3 hepatocyte marker genes (Cyp2a5, Cyp1a2, Cyp2e1, <t>Cyp7a1</t> and Lgr5) was examined using real‐time PCR analysis in D‐Hep cells during hepatic differentiation. Mean values ± SEM . were normalized to Gapdh and expressed relative to normal hepatocytes from adult mice. Superscript letters indicate significant ( p < .01) differences. p ‐Value was calculated using Tukey's honestly significant difference test. (c) Schematic diagram of the experimental procedure. (d) The cell viabilities of DFAT cells (blue), D‐Hep cells (red) and primary hepatocytes (black) were assessed by ATP assay after a 48‐hr exposure to the three compounds (acetaminophen, tamoxifen and troglitazone). p ‐Value was calculated using Student's t test (** p < .01). NS, not significant. All scale bars represent 50 μm. All quantitative data are means ± SD . ( n = 3 experiments)
Cyp2c19 Gene Sequence Data, supplied by Sequenom, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vitamin d 1α hydroxylase  (Bio-Rad)
98
Bio-Rad vitamin d 1α hydroxylase
Expression of Zone 1 and Zone 3 hepatocyte markers and function in D‐Hep cells. (a) FHS‐DFAT cells were cultured in hepatic medium for 14 days. The temporal expression of Zone 1 hepatocyte marker genes (Oct and Pck1) was examined by real‐time PCR analysis in D‐Hep cells during hepatic differentiation. (b) The temporal expression of Zone 3 hepatocyte marker genes (Cyp2a5, Cyp1a2, Cyp2e1, <t>Cyp7a1</t> and Lgr5) was examined using real‐time PCR analysis in D‐Hep cells during hepatic differentiation. Mean values ± SEM . were normalized to Gapdh and expressed relative to normal hepatocytes from adult mice. Superscript letters indicate significant ( p < .01) differences. p ‐Value was calculated using Tukey's honestly significant difference test. (c) Schematic diagram of the experimental procedure. (d) The cell viabilities of DFAT cells (blue), D‐Hep cells (red) and primary hepatocytes (black) were assessed by ATP assay after a 48‐hr exposure to the three compounds (acetaminophen, tamoxifen and troglitazone). p ‐Value was calculated using Student's t test (** p < .01). NS, not significant. All scale bars represent 50 μm. All quantitative data are means ± SD . ( n = 3 experiments)
Vitamin D 1α Hydroxylase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pqq-dependent methanol dehydrogenase mdh  (Shotgun Proteomics company)
90
Shotgun Proteomics company pqq-dependent methanol dehydrogenase mdh
Expression of Zone 1 and Zone 3 hepatocyte markers and function in D‐Hep cells. (a) FHS‐DFAT cells were cultured in hepatic medium for 14 days. The temporal expression of Zone 1 hepatocyte marker genes (Oct and Pck1) was examined by real‐time PCR analysis in D‐Hep cells during hepatic differentiation. (b) The temporal expression of Zone 3 hepatocyte marker genes (Cyp2a5, Cyp1a2, Cyp2e1, <t>Cyp7a1</t> and Lgr5) was examined using real‐time PCR analysis in D‐Hep cells during hepatic differentiation. Mean values ± SEM . were normalized to Gapdh and expressed relative to normal hepatocytes from adult mice. Superscript letters indicate significant ( p < .01) differences. p ‐Value was calculated using Tukey's honestly significant difference test. (c) Schematic diagram of the experimental procedure. (d) The cell viabilities of DFAT cells (blue), D‐Hep cells (red) and primary hepatocytes (black) were assessed by ATP assay after a 48‐hr exposure to the three compounds (acetaminophen, tamoxifen and troglitazone). p ‐Value was calculated using Student's t test (** p < .01). NS, not significant. All scale bars represent 50 μm. All quantitative data are means ± SD . ( n = 3 experiments)
Pqq Dependent Methanol Dehydrogenase Mdh, supplied by Shotgun Proteomics company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cytochrome c oxidase i gene sequence data  (Biotechnology Information)
86
Biotechnology Information cytochrome c oxidase i gene sequence data
Expression of Zone 1 and Zone 3 hepatocyte markers and function in D‐Hep cells. (a) FHS‐DFAT cells were cultured in hepatic medium for 14 days. The temporal expression of Zone 1 hepatocyte marker genes (Oct and Pck1) was examined by real‐time PCR analysis in D‐Hep cells during hepatic differentiation. (b) The temporal expression of Zone 3 hepatocyte marker genes (Cyp2a5, Cyp1a2, Cyp2e1, <t>Cyp7a1</t> and Lgr5) was examined using real‐time PCR analysis in D‐Hep cells during hepatic differentiation. Mean values ± SEM . were normalized to Gapdh and expressed relative to normal hepatocytes from adult mice. Superscript letters indicate significant ( p < .01) differences. p ‐Value was calculated using Tukey's honestly significant difference test. (c) Schematic diagram of the experimental procedure. (d) The cell viabilities of DFAT cells (blue), D‐Hep cells (red) and primary hepatocytes (black) were assessed by ATP assay after a 48‐hr exposure to the three compounds (acetaminophen, tamoxifen and troglitazone). p ‐Value was calculated using Student's t test (** p < .01). NS, not significant. All scale bars represent 50 μm. All quantitative data are means ± SD . ( n = 3 experiments)
Cytochrome C Oxidase I Gene Sequence Data, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cyp17 cdna  (Genome Systems Inc)
90
Genome Systems Inc cyp17 cdna
Expression of Zone 1 and Zone 3 hepatocyte markers and function in D‐Hep cells. (a) FHS‐DFAT cells were cultured in hepatic medium for 14 days. The temporal expression of Zone 1 hepatocyte marker genes (Oct and Pck1) was examined by real‐time PCR analysis in D‐Hep cells during hepatic differentiation. (b) The temporal expression of Zone 3 hepatocyte marker genes (Cyp2a5, Cyp1a2, Cyp2e1, <t>Cyp7a1</t> and Lgr5) was examined using real‐time PCR analysis in D‐Hep cells during hepatic differentiation. Mean values ± SEM . were normalized to Gapdh and expressed relative to normal hepatocytes from adult mice. Superscript letters indicate significant ( p < .01) differences. p ‐Value was calculated using Tukey's honestly significant difference test. (c) Schematic diagram of the experimental procedure. (d) The cell viabilities of DFAT cells (blue), D‐Hep cells (red) and primary hepatocytes (black) were assessed by ATP assay after a 48‐hr exposure to the three compounds (acetaminophen, tamoxifen and troglitazone). p ‐Value was calculated using Student's t test (** p < .01). NS, not significant. All scale bars represent 50 μm. All quantitative data are means ± SD . ( n = 3 experiments)
Cyp17 Cdna, supplied by Genome Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp cyp1a2 mm00487224 m1  (Thermo Fisher)
94
Thermo Fisher gene exp cyp1a2 mm00487224 m1
Expression of Zone 1 and Zone 3 hepatocyte markers and function in D‐Hep cells. (a) FHS‐DFAT cells were cultured in hepatic medium for 14 days. The temporal expression of Zone 1 hepatocyte marker genes (Oct and Pck1) was examined by real‐time PCR analysis in D‐Hep cells during hepatic differentiation. (b) The temporal expression of Zone 3 hepatocyte marker genes (Cyp2a5, <t>Cyp1a2,</t> Cyp2e1, Cyp7a1 and Lgr5) was examined using real‐time PCR analysis in D‐Hep cells during hepatic differentiation. Mean values ± SEM . were normalized to Gapdh and expressed relative to normal hepatocytes from adult mice. Superscript letters indicate significant ( p < .01) differences. p ‐Value was calculated using Tukey's honestly significant difference test. (c) Schematic diagram of the experimental procedure. (d) The cell viabilities of DFAT cells (blue), D‐Hep cells (red) and primary hepatocytes (black) were assessed by ATP assay after a 48‐hr exposure to the three compounds (acetaminophen, tamoxifen and troglitazone). p ‐Value was calculated using Student's t test (** p < .01). NS, not significant. All scale bars represent 50 μm. All quantitative data are means ± SD . ( n = 3 experiments)
Gene Exp Cyp1a2 Mm00487224 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Expression of Zone 1 and Zone 3 hepatocyte markers and function in D‐Hep cells. (a) FHS‐DFAT cells were cultured in hepatic medium for 14 days. The temporal expression of Zone 1 hepatocyte marker genes (Oct and Pck1) was examined by real‐time PCR analysis in D‐Hep cells during hepatic differentiation. (b) The temporal expression of Zone 3 hepatocyte marker genes (Cyp2a5, Cyp1a2, Cyp2e1, Cyp7a1 and Lgr5) was examined using real‐time PCR analysis in D‐Hep cells during hepatic differentiation. Mean values ± SEM . were normalized to Gapdh and expressed relative to normal hepatocytes from adult mice. Superscript letters indicate significant ( p < .01) differences. p ‐Value was calculated using Tukey's honestly significant difference test. (c) Schematic diagram of the experimental procedure. (d) The cell viabilities of DFAT cells (blue), D‐Hep cells (red) and primary hepatocytes (black) were assessed by ATP assay after a 48‐hr exposure to the three compounds (acetaminophen, tamoxifen and troglitazone). p ‐Value was calculated using Student's t test (** p < .01). NS, not significant. All scale bars represent 50 μm. All quantitative data are means ± SD . ( n = 3 experiments)

Journal: Genes to Cells

Article Title: Generation of metabolically functional hepatocyte‐like cells from dedifferentiated fat cells by Foxa2, Hnf4a and Sall1 transduction

doi: 10.1111/gtc.12814

Figure Lengend Snippet: Expression of Zone 1 and Zone 3 hepatocyte markers and function in D‐Hep cells. (a) FHS‐DFAT cells were cultured in hepatic medium for 14 days. The temporal expression of Zone 1 hepatocyte marker genes (Oct and Pck1) was examined by real‐time PCR analysis in D‐Hep cells during hepatic differentiation. (b) The temporal expression of Zone 3 hepatocyte marker genes (Cyp2a5, Cyp1a2, Cyp2e1, Cyp7a1 and Lgr5) was examined using real‐time PCR analysis in D‐Hep cells during hepatic differentiation. Mean values ± SEM . were normalized to Gapdh and expressed relative to normal hepatocytes from adult mice. Superscript letters indicate significant ( p < .01) differences. p ‐Value was calculated using Tukey's honestly significant difference test. (c) Schematic diagram of the experimental procedure. (d) The cell viabilities of DFAT cells (blue), D‐Hep cells (red) and primary hepatocytes (black) were assessed by ATP assay after a 48‐hr exposure to the three compounds (acetaminophen, tamoxifen and troglitazone). p ‐Value was calculated using Student's t test (** p < .01). NS, not significant. All scale bars represent 50 μm. All quantitative data are means ± SD . ( n = 3 experiments)

Article Snippet: The probes for Alb (GenBank accession no. NM_009654.3; Mm00802090_m1), Afp (GenBank accession no. NM_007423.4; Mm00431715_m1), Tat (GenBank accession no. NM_146214.3; Mm01244282_m1), Tdo2 (GenBank accession no. NM_019911.2; Mm00451269_m1), Cyp1a2 (GenBank accession no. NM_009993.3; Mm00487224_m1), Cyp2e1 (GenBank accession no. NM_021282.2; Mm00491127_m1), Cyp2a5 (GenBank accession no. NM_007812.4; Mm00487248_g1), Cyp7a1 (GenBank accession no. NM_007824.2; Mm00484150_m1), Lgr5 (GenBank accession no. NM_010195.2; Mm00438890_m1), Otc (GenBank accession no. NM_008769.3; Mm00493267_m1) and Pck1 (GenBank accession no. NM_011044.2; Mm01247058_m1) genes were obtained from TaqMan Pre‐Developed Assay Reagents (Applied Biosystems).

Techniques: Expressing, Cell Culture, Marker, Real-time Polymerase Chain Reaction, ATP Assay

Expression of Zone 1 and Zone 3 hepatocyte markers and function in D‐Hep cells. (a) FHS‐DFAT cells were cultured in hepatic medium for 14 days. The temporal expression of Zone 1 hepatocyte marker genes (Oct and Pck1) was examined by real‐time PCR analysis in D‐Hep cells during hepatic differentiation. (b) The temporal expression of Zone 3 hepatocyte marker genes (Cyp2a5, Cyp1a2, Cyp2e1, Cyp7a1 and Lgr5) was examined using real‐time PCR analysis in D‐Hep cells during hepatic differentiation. Mean values ± SEM . were normalized to Gapdh and expressed relative to normal hepatocytes from adult mice. Superscript letters indicate significant ( p < .01) differences. p ‐Value was calculated using Tukey's honestly significant difference test. (c) Schematic diagram of the experimental procedure. (d) The cell viabilities of DFAT cells (blue), D‐Hep cells (red) and primary hepatocytes (black) were assessed by ATP assay after a 48‐hr exposure to the three compounds (acetaminophen, tamoxifen and troglitazone). p ‐Value was calculated using Student's t test (** p < .01). NS, not significant. All scale bars represent 50 μm. All quantitative data are means ± SD . ( n = 3 experiments)

Journal: Genes to Cells

Article Title: Generation of metabolically functional hepatocyte‐like cells from dedifferentiated fat cells by Foxa2, Hnf4a and Sall1 transduction

doi: 10.1111/gtc.12814

Figure Lengend Snippet: Expression of Zone 1 and Zone 3 hepatocyte markers and function in D‐Hep cells. (a) FHS‐DFAT cells were cultured in hepatic medium for 14 days. The temporal expression of Zone 1 hepatocyte marker genes (Oct and Pck1) was examined by real‐time PCR analysis in D‐Hep cells during hepatic differentiation. (b) The temporal expression of Zone 3 hepatocyte marker genes (Cyp2a5, Cyp1a2, Cyp2e1, Cyp7a1 and Lgr5) was examined using real‐time PCR analysis in D‐Hep cells during hepatic differentiation. Mean values ± SEM . were normalized to Gapdh and expressed relative to normal hepatocytes from adult mice. Superscript letters indicate significant ( p < .01) differences. p ‐Value was calculated using Tukey's honestly significant difference test. (c) Schematic diagram of the experimental procedure. (d) The cell viabilities of DFAT cells (blue), D‐Hep cells (red) and primary hepatocytes (black) were assessed by ATP assay after a 48‐hr exposure to the three compounds (acetaminophen, tamoxifen and troglitazone). p ‐Value was calculated using Student's t test (** p < .01). NS, not significant. All scale bars represent 50 μm. All quantitative data are means ± SD . ( n = 3 experiments)

Article Snippet: The probes for Alb (GenBank accession no. NM_009654.3; Mm00802090_m1), Afp (GenBank accession no. NM_007423.4; Mm00431715_m1), Tat (GenBank accession no. NM_146214.3; Mm01244282_m1), Tdo2 (GenBank accession no. NM_019911.2; Mm00451269_m1), Cyp1a2 (GenBank accession no. NM_009993.3; Mm00487224_m1), Cyp2e1 (GenBank accession no. NM_021282.2; Mm00491127_m1), Cyp2a5 (GenBank accession no. NM_007812.4; Mm00487248_g1), Cyp7a1 (GenBank accession no. NM_007824.2; Mm00484150_m1), Lgr5 (GenBank accession no. NM_010195.2; Mm00438890_m1), Otc (GenBank accession no. NM_008769.3; Mm00493267_m1) and Pck1 (GenBank accession no. NM_011044.2; Mm01247058_m1) genes were obtained from TaqMan Pre‐Developed Assay Reagents (Applied Biosystems).

Techniques: Expressing, Cell Culture, Marker, Real-time Polymerase Chain Reaction, ATP Assay

Cyp1a2‐mediated drug metabolism in D‐Hep cells. (a) FHS‐DFAT cells and GFP‐DFAT cells (Ctrl) before (D0) and after (D14) induction of differentiation and primary hepatocytes (HC) were subjected to immunostaining with anti‐Cyp1a2 (red) antibodies. Nuclei were stained with Hoechst 33342 (blue). (b) Cyp1a2 activity in FHS‐DFAT cells and HCs was measured. p ‐Value was calculated using Student's t test (* p < .05, ** p < .01). (c) The cell viabilities of DFAT cells (blue), D‐Hep cells (red) and HCs (black) were assessed by ATP assay after a 48‐hr exposure to different concentrations of APAP. p ‐Value was calculated using Tukey's honestly significant difference test compared to D‐Hep cells. (** p < .01). (d) GSH content and GPT activity in cells treated as in C. p ‐Value was calculated using Tukey's honestly significant difference test (* p < .05). NS, not significant. Scale bars represent 50 μm. All quantitative data are means ± SD . ( n = 3 experiments)

Journal: Genes to Cells

Article Title: Generation of metabolically functional hepatocyte‐like cells from dedifferentiated fat cells by Foxa2, Hnf4a and Sall1 transduction

doi: 10.1111/gtc.12814

Figure Lengend Snippet: Cyp1a2‐mediated drug metabolism in D‐Hep cells. (a) FHS‐DFAT cells and GFP‐DFAT cells (Ctrl) before (D0) and after (D14) induction of differentiation and primary hepatocytes (HC) were subjected to immunostaining with anti‐Cyp1a2 (red) antibodies. Nuclei were stained with Hoechst 33342 (blue). (b) Cyp1a2 activity in FHS‐DFAT cells and HCs was measured. p ‐Value was calculated using Student's t test (* p < .05, ** p < .01). (c) The cell viabilities of DFAT cells (blue), D‐Hep cells (red) and HCs (black) were assessed by ATP assay after a 48‐hr exposure to different concentrations of APAP. p ‐Value was calculated using Tukey's honestly significant difference test compared to D‐Hep cells. (** p < .01). (d) GSH content and GPT activity in cells treated as in C. p ‐Value was calculated using Tukey's honestly significant difference test (* p < .05). NS, not significant. Scale bars represent 50 μm. All quantitative data are means ± SD . ( n = 3 experiments)

Article Snippet: The probes for Alb (GenBank accession no. NM_009654.3; Mm00802090_m1), Afp (GenBank accession no. NM_007423.4; Mm00431715_m1), Tat (GenBank accession no. NM_146214.3; Mm01244282_m1), Tdo2 (GenBank accession no. NM_019911.2; Mm00451269_m1), Cyp1a2 (GenBank accession no. NM_009993.3; Mm00487224_m1), Cyp2e1 (GenBank accession no. NM_021282.2; Mm00491127_m1), Cyp2a5 (GenBank accession no. NM_007812.4; Mm00487248_g1), Cyp7a1 (GenBank accession no. NM_007824.2; Mm00484150_m1), Lgr5 (GenBank accession no. NM_010195.2; Mm00438890_m1), Otc (GenBank accession no. NM_008769.3; Mm00493267_m1) and Pck1 (GenBank accession no. NM_011044.2; Mm01247058_m1) genes were obtained from TaqMan Pre‐Developed Assay Reagents (Applied Biosystems).

Techniques: Immunostaining, Staining, Activity Assay, ATP Assay